Transgenic Mouse Technology: Principles and Methods
WEBOur group has generated several lines of transgenic mice that phenocopy many human reproductive diseases. In the following sections, we will discuss general principles of transgenic mouse technology and provide detailed methods in later sections.
Temporal BMP4 effects on mouse embryonic and extraembryonic development …
WEB1 day ago · The developing placenta, which in mice originates through the extraembryonic ectoderm (ExE), is essential for mammalian embryonic development. Yet unbiased characterization of the differentiation ...
A neuroscientist’s guide to transgenic mice and other genetic tools
WEBTopics covered include types of traditional transgenic mouse models (knockout, knock-in, reporter lines), inducible systems (Cre-loxP, Tet-On, Tet-Off) and cell- and circuit-specific systems (TetTag, TRAP, DIO-DREADD).
Crosstalk between the embryo and the developing placenta relies …
WEB1 day ago · Jobs. In mammals, signalling between the placenta and the embryo is essential for successful development. Here, a quantitative, time-resolved model describes the coordinated formation of the mouse ...
Tinkering with genes and embryos: the multiple invention of transgenic ...
WEBJan 27, 2020 · Genetically modified or ‘transgenic’ mice are a routine experimental tool in biomedical research, commonly produced by injecting DNA into one-cell embryos.
Establishment of an integrated automated embryonic ... - Nature
WEBJun 3, 2021 · Finally, we produced transgenic (traditional DNA injection and piggyBac Transposon system) and knock-in (genomic editing) mice using our newly developed Integrated Automated Embryo...
Generation of Transgenic Mice - PMC - National Center for …
WEBTo generate transgenic mice successfully, every step is critical, from designing the transgenic construct to embryo transfer. Preparing a clean DNA sample is a vital step because it affects the health of the embryo and the DNA integration efficiency.
Temporal BMP4 effects on mouse embryonic and extraembryonic development
WEBHowever, commencing at embryonic day 7.5, embryo-derived BMP4 restricts the primordial germ cell pool size by favouring differentiation of their extraembryonic mesoderm precursors towards an allantois fate. ExE and embryonic tissues are therefore entangled in time, space and signalling axes, highlighting the importance of their integrated ...
Transgenic mouse technology: principles and methods
WEBThis technology now referred to as transgenic mouse technology has revolutionized virtually all fields of biology and provided new genetic approaches to model many human diseases in a whole animal context.
Intravital imaging of mouse embryos | Science - AAAS
WEBApr 10, 2020 · In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)–derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta.